How It Works (label or sandwich-based)

Lectin Array 70

RayBio® Lectin Array 70. Detects glycan profiles using 70 lectins. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Label-based
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications



The Raybiotech lectin array uses standard glass slides each spotted with 14 wells of identical lectin arrays. Each lectin, together with the positive controls is arrayed in duplicate. The slides each come with a 16-well removable gasket which allows for the process of 14 samples using one slide. Four slide slides can be nested into a tray, which matches a standard microplate and allows for automated robotic high throughput process of 56 arrays simultaneously. The RayBiotech lectin array provides a powerful new tool for glycosylation determination, drug discovery and biomarker development; all with limited samples volumes required.

Product Feature

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 14 samples on each slide
  • Suitable for high-throughput assays

Lectin Names

AAA, AAL, ACG, ACL, ASA, BanLec, BC2L-A, BC2LCN, BPA, Calsepa, CGL2, CNL, Con A, DBA, Discoidin I, Discoidin II, DSA, ECA, EEL, F17AG, Gal1, Gal1-S, Gal2, Gal3, Gal3C-S, Gal7-S, Gal9, GNA, GRFT, GS-I, GS-II, HIHA, Jacalin, LBA, LCA, LEA, Lentil, Lotus, LSL-N, MAA, Malectin, MOA, MPL, NPA, Orysata, PA-IIL, PA-IL, PALa, PHA-E, PHA-L, PHA-P, PNA, PPL, PSA, PSL1a, PTL, RS-Fuc, SAMB, SBA, SJA, SNA-I, SNA-II, STL, UDA, UEA-I, UEA-II, VFA, VVA, WFA, WGA

Application Data/Notes

Application 1 Detection of Glycans on HRP
See Image/s

After biotinylation of HRP we can determine what kind of glycans are conjugated to HRP by using the RayBiotech lectin array. Lectins, GNA, HHA, NPA showed strong signals after incubation with 0.33 ug/mL Biotin-HRP followed detection by streptavidin-fluorescence-dye (Figure A, B and C). The fluorescence signals from GNA, HHA and NPA can be blocked in a concentration depend manner by HRP itself (Figure A and B), which means that these fluorescence signals were generated based on the binding between HRP and the three lectins. As we know, GNA, HHA and NPA lectins specifically bind to mannose which indicates that HRP contains mannose. By adding increasing amount of mannose, the signal from GNA, HHA and NPA can be reduced (Figure A and C). The reduction signals from increasing concentrations of mannose confirms that HRP protein contains mannose in its glycocalyx. Lectin VVA binds to the streptavidin-fluorescence dye.
Application 2 Profile of Serum Samples
See Image/s

Using the lectin array, we can discover the different glycoprotein profiles of the serum samples or cell lysates from patient cohorts versus a control group. Below images showed the profiles of the glycans from serum samples detected by Biotin-anti-human IgG and Fluorescence dye-streptavidin.
Suggested Applications
  • Identify and profile the glycans in their samples
  • Determine whether their biomarker of interest has glycan moieties
  • Find specific glycan binding ligands in biological samples
Other Applications
Quantitative analysis of lectin-glycoprotein interactions. Example: a concentration series of glycoproteins detected with the lectin array could reveal concentration dependent effects of lectin-glycan binding.

Determine the profile of bacterial cell-surface glycans. Example: Cell lysate from bacteria can be Biotinylated and hybridized to the lectin array. Analysis of the binding pattern and correlation with the known carbohydrate-binding specificities of the lectins can determine the glycans on the cell membrane.
Kit Components
  • Dialysis Vials
  • Labeling Reagent
  • Labeling Buffer
  • Stop Solution
  • Lectin Array Glass Slide Assembly
  • Sample Diluent
  • 20X Wash Buffer I
  • 20X Wash Buffer II
  • Cy3 equivalent dye-conjugated Streptavidin
  • Slide Washer/Dryer
  • Adhesive device sealer
  • Floating Dialysis Rack
  • Manual
Other Materials Required
  • Detection antibodies of interest (For sandwich-based method only)
  • Orbital shaker
  • Laser scanner for fluorescence detection
  • Aluminum foil
  • 1.5ml Polypropylene microcentrifuge tubes
  • KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
  • Plastic or glass containers, beaker, stir plate and stir bar
  • Pipettors, pipette tips, ddH2O and other common lab consumables
Protocol Outline
  1. Dry the glass slide
  2. Block array surfaces
  3. Incubate samples (samples need to be biotinylated for the label-based approach)
  4. For the the sandwich-based principle, incubate with a detection antibody cocktail
    For the label-based principle, incubate the labeled-streptavidin.
  5. Incubate with Cy3 Equivalent Dye-Streptavidin
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis


Upon receipt, all components of the Raybiotech Lectin Array 70 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.
These arrays work well, but data analysis is not always reliable. This may be due to the user, however. Shipping is fast and the company is very accommodating. Protocol is two days long, and it is not possible to analyse live cells due to the necessity of dry chips.

Blood Center of Wisconsin
Good assay, needed a lot of help from tech support to run successfully on account of being first time use.

Abclinical Discovery
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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