Monkeypox Virus Real-Time PCR Kit

RayBiotech Monkeypox Virus (MPXV) PCR Nucleic Acid Detection Kit is a real-time PCR assay for the detection of MPXV DNA in a liquid sample. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV.

$420.00  
PCR-MPXV
+

Assay Format

Sensitivity:
4 copies / µl
Specificity:
Does not cross react with cowpox, camelpox or vaccinia virus. Features dUTP-UDG system to prevent carry-over contamination.
Number of Targets Detected:
1
Species Detected:
  • Virus
Compatible Sample Types:
  • DNA
Method of Detection:
PCR
Quantitative/Semi-Quantitative:
  • Qualitative

Product Specifications

Size:
96 tests

Protein Information

Accession Number:
  • O57108
Gene Symbols:
  • J2R
Protein Name & Synonyms:
J2R (J2L)

Introduction

While Monkeypox viruses (MPXV) are usually zoonotic diseases (generally transmitted from animal to human), this MPXV outbreak is seeing higher levels of presumptive human to human spread for reasons that are currently not well understood. This new MPXV isolate is nearly identical to a recent outbreak in the UK, differing by less than 100 bases out of over 197,000 nucleotides. RayBiotech developed this Monkeypox Virus PCR Kit as a ready to use PCR assay for the detection of MPXV DNA to help the research community better test, evaluate, and understand this current viral strain. It does this by using primer sets specific for the J2R gene unique to the current strain of circulating MPXV (GenBank: ON563414.3) and can be measured with most common qPCR setups.

PCR Kit Amplification Plots

PCR amplification plot showing the high specificity of this product Amplification plot showing the high reproducibility of this product

Product Features

  • Monkeypox virus specific / no cross-reacitvity with other Orthopox viruses.
  • Hot-start Taq DNA Polymerase with high efficiency and fidelity.
  • Double quencher probe ensures enhanced specificity.
  • UDG and dUTP system minimizes carry-over contamination.
  • ROX reference dye improves accuracy and reproducibility.

Kit Components

Component Ingredients Specification Quantity
2x PCR Reaction Solution Buffer, dNTP’s, enzyme, ROX reference dye 1000 µL / tube 1 tube
Primers and Probe Oligonucleotides 500 μl / tube 1 tube
Positive Control Synthetic Monkeypox Virus Genomic DNA 50 µL / tube 1 tube
Negative Control Nuclease-free water 500 µL / tube 1 tube

Other Materials Required

  • Sample DNA purification; can be Trizol based methods, or common commercially available purifications kits.
  • DNA preservation solution
  • Fluorescence PCR instrument capable of reading FAM or equivalent channel (494 nm maximum absorption, 518 nm maximum emission).
  • Vortex Mixer
  • Microcentrifuge
  • Pipettes
  • Sterile nuclease-free pipette tips (barrier tips recommended) and microfuge tubes
  • Compatible PCR Plate

Contact our technical support team for questions about compatibility: [email protected] 

Storage/Stability

The Monkeypox PCR kit is shipped on dry ice and can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.