Adrenomedullin (ADM or AM) consists of 52 amino acids, and is a potent hypotensive and vasodilatator agent. It was initially isolated in 1993 from a pheochromocytoma, a tumor of the adrenal medulla. ADM is found in blood in a considerable concentration and may function as a hormone related to the physiologic control of fluid and electrolyte homeostasis. It has also been reported that ADM stimulates the growth of new blood vessels (angiogenesis) and increasing the tolerance of cells to oxidative stress and hypoxic injury.
Product Features
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Typical Data
Standard Curve
Application Notes
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
HRP-Streptavidin
TMB One-Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
