Mouse Anti-5-Methylcytidine

Mouse Anti-5-Methylcytidine

DS-MB-00003
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Antigen Information

Protein Name & Synonyms:
5-Methylcytidine

Antibody Specifications

Applications:
Immunohistology - Frozen, Flow Cytometry, Immunoblotting, Immunofluorescence, Methylated DNA Immunoprecipitation, Immunohistology - Paraffin
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Broad, Purified IgG - liquid, Human, Mouse, Rat

Specificity

This product recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression.

Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.

This antibody has been reported for use in Methylated DNA immunoprecipitation (MeDIP).

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Preservative:
0.01%Thiomersal
This product
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag 14 myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.