Mouse Anti-Anthrax PA

Mouse Anti-Anthrax PA

$488.00
DS-MB-02603
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Antigen Information

Protein Name & Synonyms:
Anthrax PA
Target Species:
  • Bacterial

Antibody Specifications

Applications:
ELISA, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Species Reactivity:
Bacterial

Specificity

This product recognizes the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol.

This product does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensis or Toxoplasma gondii. The antibody recognises both the 83kDa and 63kDa truncated form of PA.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Highly purified protective antigen of Bacillus anthracis.
Preservative:
0.09%Sodium Azide (NaN3)
This product
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.