Protein Name & Synonyms:
Immunohistology - Frozen, Flow Cytometry, Immunoprecipitation
IgG concentration 0.5 mg/ml
This product recognizes the chicken CD3 cell surface antigen, a complex of at least three polypeptides of 20, 19 and 17kD expressed by T cells. The antibody also co-precipitates a 90kD heterodimer from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of 40kD and 50 kD.
In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Borate buffered saline
Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
Lymph node cells from immunised BALB/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.