Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH) provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. RayBiotech's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Mouse Monoclonal Antibody (Mab)
WB: 1:1; 000
HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody.
2-8°C (short-term); -20°C (long-term)
12 months from the date of shipment when stored properly.