Mouse Anti-Human C4d (Neoantigen)

Mouse Anti-Human C4d (Neoantigen)
CODE:  DS-MB-02780

Antigen Information

Protein Name & Synonyms:
C4d (Neoantigen)
Target Species:
  • Human

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA, Flow Cytometry
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Human

Specificity

This product recognizes a neoantigen on the secreted protein complement component 4d (C4d). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b❏'s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. The short half life of C4d means that it is present in serum at high enough concentrations to make it a useful marker for classical complement activation.

C4 has been linked to susceptibility to systemic lupus erythematosous.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Borate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Purified human C4d
Preservative:
0.1% Sodium Azide (NaN3)