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Product Specifications

Size:

0.1 mg

Protein Information

Protein Name & Synonyms:

C4d

Target Species:

Human

Antibody Specifications

Applications:

Immunohistology - Frozen, ELISA (dilution: 1/250, 1/600), Immunofluorescence, Immunohistology - Paraffin, Western Blotting

Concentration (lot specific):

IgG concentration 1.0 mg/ml

Species Reactivity:

Human

Specificity

This product recognizes the secreted protein complement component 4d (C4d). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b❏'s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4d❏'s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation.

C4 has been linked to susceptibility to systemic lupus erythematosous.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:

Purified IgG - liquid

Formulation:

Borate buffered saline

Format Type:

Purified

Preparation

Immunogen:

Native, from human plasma

Preservative:

0.09%

Sodium Azide (NaN₃)

Expiration:

12 months from the date of shipment when stored properly.