Protein Name & Synonyms:
ELISA, Flow Cytometry, Radioimmunoassays
IgG concentration 1.0 mg/ml
This product recognizes an extracellular domain of the 55kD human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75kD TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes.
This product may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application.
This product is routinely tested in flow cytometry on human peripheral blood monocytes.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Purified human tumour necrosis factor receptor type 1
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.