Protein Name & Synonyms:
Flow Cytometry, Functional Assays, Western Blotting
IgG concentration 1.0mg/ml
This product detects a band of approximately 45-60kDa in 293 human fibroblastoid cell lysates under non-reducing conditions.
This product specifically recognizes the Ig domain D2 of human CD147, otherwise known as neurothelin, a type I transmembrane glycoprotein and member of the immunoglobulin gene superfamily, broadly expressed on peripheral blood cells, endothelial cells, and increased on T cells following activation. CD147 is also strongly expressed on small lung cancer cells and hepatocellular carcinoma.
Biological properties of CD147 include the regulation of T cells, integrin functions and cell adhesion, the stimulation of metalloproteinase (MMP) production by fibroblasts, the promotion of astrocyte outgrowths, and roles in tumour progression, neuronal system function and reproduction.
This product inhibits anti-CD3-induced T cell activation, dependent upon antibody concentration(1 ).
Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
CD147Rg, consisting of the CDNA coding for the hinge region, CH2-and CH3 domain of human IgG1.
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.