Protein Name & Synonyms:
Flow Cytometry, Immunoprecipitation
IgG concentration 1.0 mg/ml
This product recognizes CD16, a 50-65kD cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.
CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. This product has been reported to block the binding of IgG to the CD16 molecule, see Tamm, A. and Schmidt, R.E. for details.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Normal human peripheral blood granulocytes
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.