Protein Name & Synonyms:
Flow Cytometry, Functional Assays, Immunohistology - Paraffin, Western Blotting
IgG concentration 1.0 mg/ml
This product recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. This antibody recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting, see Li, S.L. et al. for details.
CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.
The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. This product is reported to partically block binding of IGF-I and IGF-II to CD221, see Li, S.L. et al for details.
Store at -20 °C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Quality Control (Contaminants):
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.