Protein Name & Synonyms:
Immunohistology - Frozen, Flow Cytometry, Immunoprecipitation
IgG concentration 1.0 mg/ml
Human, This antibody is reported to cross react to Cynomolgus monkey and bovine
This product recognizes human CD62L, also known a L-selectin, a 74-95kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62-P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes.
CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naive lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains.
The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
PHA stimulated lymphoblasts.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.