Mouse Anti-Human CKB

Mouse Anti-Human CKB Antibody
CODE:  130-10124

Antigen Information

Accession Number:
  • P12277
Antigen Catalog Number:
230-00039
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (recommended work dilution= 1: 40,000)
Preparation:
Immunogen was recombinant protein derived from Human CKB. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein A/G affinity chromatography.
Reconstitution:
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1 x PBS to a final concentration of 1 mg/ml.

Description

Creatine phosphokinase, also known as creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In cells, the "cytosolic" CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).

Specificity

The antibody specifically binds to human CKB Cross reactivity with mouse and rat were not tested.

Storage

Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.