Mouse Anti-Human CRF

Mouse Anti-Human CRF

$488.00
DS-MB-01055
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Antigen Information

Protein Name & Synonyms:
CRF
Target Species:
  • Human

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA (dilution: 1/100, 1/7000), Immunohistology - Paraffin
Concentration:
1mg/ml
Species Reactivity:
Human
Immunohistology:
*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.

Specificity

This product is specific for corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.

The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.

Averages 10(e)-9 M with native and modified CRF.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified Ig - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.