Protein Name & Synonyms:
ELISA (dilution: 1/1000, 1/2000), Western Blotting
IgG concentration 1.0mg/ml
This product recognizes alpha A crystallin, a 20kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB4, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index.
Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
E. coli-derived recombinant protein corresponding to full-length (aa1-173) human crystallin alpha A.
0.09% Sodium Azide (NaN3)
Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.