Protein Name & Synonyms:
Immunohistology - Frozen, ELISA (dilution: 1/100, 1/500), Flow Cytometry, Western Blotting
Concentration (lot specific):
IgM concentration 1.0 mg/ml
This product recognizes human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.
In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgM - liquid
Phosphate buffered saline
Human monocytes and U937 cell line
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
12 months from the date of shipment when stored properly.