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Mouse Anti-Human Fibroblasts/Epithelial cells [+RPE]

Mouse Anti-Human Fibroblasts/Epithelial cells [+RPE]

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DS-MB-03293
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Antigen Information

Protein Name & Synonyms:
Fibroblasts/Epithelial cells
Target Species:
  • Human

Antibody Specifications

Applications:
Flow Cytometry
Reconstitution:
Reconstitute in 0.25 ml disilled water
Species Reactivity:
Human

Specificity

This antibody recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.

Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.

In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes

This product is routinely tested in flow cytometry on the KG1 cell line.

Storage/Stability

Store at +4 °C. DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute in 0.25 ml disilled water

Preparation

Immunogen:
Human foreskin fibroblasts
Preservative:
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line
  1. Srivastava, Girish K., et al. "Flow cytometry assessment of the purity of human retinal pigment epithelial primary cell cultures." Journal of immunological methods 389.1 (2013): 61-68.
    Species: Human
    Sample type:

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.