Protein Name & Synonyms:
Fibroblasts/Epithelial cells
Applications:
Flow Cytometry
Reconstitution:
Reconstitute in 0.25 ml disilled water
Species Reactivity:
Human
This antibody recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes
This product is routinely tested in flow cytometry on the KG1 cell line.
Store at +4 °C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute in 0.25 ml disilled water
Immunogen:
Human foreskin fibroblasts
Preservative:
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line
Expiration:
12 months from the date of shipment when stored properly.