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Antigen Information
Protein Name & Synonyms:
Fibroblasts/Epithelial cells
Target Species:
- Human
Antibody Specifications
Applications:
Immunohistology - Frozen, Flow Cytometry, Immunoprecipitation
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Human
Specificity
This antibody recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
This product is routinely tested in flow cytometry on the KG1 cell line.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
This product is routinely tested in flow cytometry on the KG1 cell line.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Immunogen:
Human foreskin fibroblasts
Preservative:
| 0.09% | Sodium Azide |
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
