Protein Name & Synonyms:
Immunohistology - Frozen, Flow Cytometry, Immunoprecipitation
Concentration (lot specific):
IgG concentration 1.0 mg/ml
This antibody recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
This product is routinely tested in flow cytometry on the KG1 cell line.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Human foreskin fibroblasts
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line
12 months from the date of shipment when stored properly.