Mouse Anti-Human Hexokinase 1

Mouse Anti-Human Hexokinase 1

$550.00
DS-MB-03303
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Antigen Information

Protein Name & Synonyms:
Hexokinase 1
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 1/1000, 1/2000), Western Blotting
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Human

This product detects a band of approximately 102kDa in HepG2 cell lysates.

Specificity

This product specifically recognizes Hexokinase 1, a 102kDa major allosteric enzyme of the glycolytic pathway, which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).

Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type I to IV), which is expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic hemolytic anaemia.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Recombinant human hexokinase 1 (amino acids 1-917) purified from Escherichia coli.
Preservative:
0.1% Sodium Azide (NaN3)
This product
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.