Protein Name & Synonyms:
ELISA, Western Blotting
IgG concentration 1.0mg/ml
This product recognizes the lectin-like oxidised low-density lipoprotein (LDL) receptor (LOX-1), a 31kDa protein of the C-type lectin superfamily. This receptor mediates the recognition, internalisation and degradation of oxidised LDL. It is a Type II membrane protein with a typical C-type lectin structure at the extracellular C-terminus which recognizes the ligand. LOX-1 activation by oxidised LDL causes endothelial changes such as decreased nitric oxide release and an increased expression of adhesion molecules. LOX-1 also binds activated platelets and apoptotic cells. The expression of LOX-1 is induced by proatherogenic conditions such as hyperlipidemia, hypertension and diabetes and as such appears to contribute to the pathogenesis of vascular disorders, particularly atherosclerosis. It can be cleaved by an unknown protease at the extracellular juxtamembrane region to release the soluble form (aa58-273) of LOX-1, recognized by this product.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Recombinant soluble LOX-1 expressed in E.coli.
0.09% Sodium Azide (NaN3)
Spleen cells from immunised Balb/c were fused with cells of the Sp2/0 myeloma cell line.