Mouse Anti-Human MAdCAM-1
Mouse Anti-Human MAdCAM-1 (0.5 mg)
Protein Name & Synonyms:
ELISA (dilution: 1/10 , 1/100), Flow Cytometry, Functional Assays, Immunohistology - Paraffin, Western Blotting
IgG concentration 1.0 mg/ml
This product detects a band of approximately 60kDa.
This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections.
This product recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but This antibody reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by This product This product is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7, see Leung, E. et al. for details.
Store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Recombinant soluble MAdCAM-1-fc fusion protein
Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.