Protein Name & Synonyms:
Immunohistology - Paraffin, Western Blotting
This product detects a band of approximately 63kDa.
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
This product recognizes the active form of human matrix metalloproteinase 9 (MMP-9). The MMP's are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMP's are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-9 is a gelatinase, cleaving type IV collagen and gelatin. The gelatinases have an additional gelatin-binding domain inserted in the catalytic domain. MMP-9, alongside other MMPs, plays a role in normal tissue remodeling such as embryonic development, ovulation, mammary gland involution and wound healing. It is important in the early stages of tumor invasion as it degrades the type IV collagen in the basement membrane.
This product recognizes only the active form of MMP-9. It does not react with the MMP-9 proenzyme or the active or proenzyme forms of MMP-2.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Ovalbumin conjugated synthetic peptide corresponding to a region within the N-terminus of human MMP-9.
0.09% Sodium Azide (NaN3)
Spleen cells from immunised Balb/c mice were fused with cells of the Ag8563 myeloma cell line.