Human PIK3R1 monoclonal antibody (100 ug)
$376.00
In stock
Code:
101-11255
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Product Description

Specifications

Size 100 ug
Protein Name / Synonyms GRB1, p85, p85-ALPHA
Accession Number AAH30815
Gene Symbols PIK3R1
Gene Id 5295
Applications WB, ELISA, IP, PLA
Isotype kappa, Igg1
Immunogen Sequence MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPK PPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDT ADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKY GFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSK YQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEY TRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIE KFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLK KQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVR QKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSN RNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHC VINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSL NVTLAYPVYAQQRR
Gene Names PIK3R1
More Information Specificity

Human
Host Species Mouse
Clonality Monoclonal
Species Human, Mouse, Rat

Synonyms

GRB1, p85, p85-ALPHA

Description

Mouse monoclonal antibody raised against a full length recombinant PIK3R1.

Source

Specificity
Human

Gene Information

Gene Accession #
BC030815

Preparation

Storage Buffer
1x PBS, pH 7.2

Western Blot detection against Immunogen (75.68 KDa).
PIK3R1 monoclonal antibody. Western Blot analysis of PIK3R1 expression in Raw 264.7 .
PIK3R1 monoclonal antibody. Western Blot analysis of PIK3R1 expression in PC-12 .
PIK3R1 monoclonal antibody. Western Blot analysis of PIK3R1 expression in NIH/3T3 .
PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa .
Western Blot analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody . Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa). Lane 2: Non-transfected lysate.
Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 monoclonal antibody.
Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.

Storage

Storage Recommendations
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Expiration:
12 months from the date of shipment when stored properly.
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