Protein Name & Synonyms:
ELISA, Western Blotting
IgG concentration 1 mg/ml
This product recognizes S100 protein derived from human brain tissue. It is a 21kDa acidic calcium-binding protein synthesised by astroglial cells. It can be found predominantly as two isoforms, the alpha-beta heterodimer (S100a) and the beta-beta homodimer (S100b). The S100 family of proteins have been implicated in a large variety of intracellular and extracellular regulatory activities, such as cellular energy metabolism, cytoskeleton modification, cell proliferation and differentiation.
When glial cells are damaged, S100 protein is leaked into the extracellular matrix and cerebrospinal fluid, further releasing into the bloodstream. S100 protein can be used as a sensitive and reliable marker for nervous system damage, such as after brain damage or acute stroke.
This product recognizes both S100a (alpha-beta) and S100b (beta-beta).
This product is sensitive to EDTA and other bivalent-binding agents. Improved performance can be obtained in the presence of 5mM CaCl2 in assay buffers.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Human brain S100 protein
Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 myeloma cell line.