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Product Description

Specifications

Size	400 µl
Protein Name / Synonyms	SOX2
Accession Number	P48431
Gene Symbols	SOX2
Gene Id	6657
Concentration (lot specific)	0.500 mg/ml
Preparation	SOX2 recombinant protein is used to produce this monoclonal antibody.
Clonality	Monoclonal
Species	Human

Introduction

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Specificity

Other Names

SOX2; Transcription factor SOX-2

NCBI Accession #

NP_003097.1

Format

Type

Purified Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

34310

Recommended Dilutions

IF: 1:100; WB: 1:200-2000; IHC: 1:50-100; FC: 1:10-50

	Fluorescent image of A549 cell stained with SOX2 Antibody(Cat# 102-10271/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).SOX2 immunoreactivity is localized to Nucleus significantly.
	Western blot analysis of SOX2 Antibody (Cat# 102-10271) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.
	Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat# 102-10271). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat# 102-10271), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
	Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10271 SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.