Protein Name & Synonyms:
ELISA (dilution: 1/1000, 1/2000), Western Blotting
IgG concentration 1.0 mg/ml
This product detects a band of approximately 121 kDa in Ramos cell lysates.
This product specifically recognizes human TLR7 (toll-like receptor 7), a 121 kDa type I transmembrane protein which belongs to the toll-like receptor (TLR) family, and is predominantly expressed in the spleen, lung and placenta.
TLRs are highly conserved proteins which play a key role in activation of innate immunity, through the recognition of distinct molecular patterns expressed by infectious agents. TLR7 recognizes and interacts with single-stranded RNA (ssRNA) derived from viruses such as influenza , HIV-1 (human immunodeficiency virus type I) and Sendai, and some siRNAs, resulting in the activation of the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway, and the production of proinflammatory signaling molecules.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Recombinant human TLR7 (amino acids 451-500) purified from Escherichia coli.
0.09% Sodium Azide (NaN3)
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.