Mouse Anti-Human TNF alpha

Mouse Anti-Human TNF alpha Antibody

$51.51  
RB-01-0006-20
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Antigen Information

Accession Number:
  • NM_000595
Target Species:
  • Human

Antibody Specifications

Applications:
Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 ng/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 ?g/mL with the appropriate secondary reagents to detect human TNF-alpha.,
Preparation:
Immunogen was full recombinant protein derived from human TNFalpha. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Reconstitution:
Lyophilized mouse anti-human TNFalpha monoclonal antibody is stable at 4 °C and below -18 °C. Supplied as 100 µg of purified antibody. Reconstitute with sterile PBS. If 1 ml of PBS is used, the antibody concentration will be 100 ?g/ml. Please avoid freeze-thaw cycles.
Species Reactivity:
Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.

Description

Tumor necrosis factor alpha (TNFalpha), also known as lymphotoxin and cachectin, is a pleiotropic cytokine. TNFalpha is a smaller cytokine with a molecular weight of 26 kDa (transmembrane) and 17 kDa (soluble) that binds to the same receptors producing a vast array of effects similar to those of TNFbeta. Both TNFalpha and TNFbeta share 30% amino acid homology and have similar biological activities. TNFalpha is produced primarily by monocytes, neutrophils and macrophages. It is found in synovial cells and macrophages in the tissues. TNFalpha and TNFbeta can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 (55 kDa) and 2 (75 kDa) to be killed, with the p55 receptor mediating the cytotoxic response.

Specificity

Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.

Storage

Lyophilized samples are stable for a year at least from date of receipt when stored at -20° C to -70° C. Upon reconstitution, the antibody can be stored at 2° - 8° C for at least a month without detectable loss of activity. Minimize freezing and thawing.

References

  • Nedwin, G.E., et al. 1985. Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucl. Acids Res. 13: 6361-6373.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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