Protein Name & Synonyms:
Troponin I Cardiac (Dephosphorylated)
ELISA, Western Blotting
IgG concentration 1 mg/ml
This product recognizes the dephosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kDa subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death.
Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts.
This product recognizes the dephosphorylated form of cTnI. It specifically recognizes amino acid residues 20-24 on cTnI. The antibody will recognize free dephosphorylated cTnI or dephosphorylated cTnI in complex with other troponin components. This product does not cross react with phosphorylated cTnI or with skeletal TnI.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Dephosphorylated form of human cardiac Troponin I.
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.