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Antigen Information
Protein Name & Synonyms:
Vimentin [+Biotin]
Target Species:
- Human
Antibody Specifications
Applications:
Immunohistochemistry.
Concentration:
1mg/ml in PBS (after reconstitution)
Host Species:
Mouse
Reconstitution:
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Species Reactivity:
Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.
Clonality:
Monoclonal
Type
Mouse Anti Human Monoclonal Antibody.
Introduction
Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.
Titer
For intra-cellular staining, use 5-10µl/1,000,000 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent.
Storage/Stability
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Preparation
Immunogen
Purified Human Vimentin
More Information
Shipping Conditions
Antibody is shipped lyophilized at ambient temperature.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
