Mouse Anti-IgG2a neg. control

Mouse Anti-IgG2a neg. control

DS-MB-03585
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Antigen Information

Protein Name & Synonyms:
IgG2a
Target Species:
  • Negative Control

Antibody Specifications

Applications:
Flow Cytometry
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Negative Control

Specificity

This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.

This antibody may not be suitable for intracellular staining on some cell types.

This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.

This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.

Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

Storage/Stability

Store at -20 °C only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Quality Control (Contaminants):
<0.01 EU/mg
Format Type:
Low Endotoxin

Preparation

Immunogen:
Activated rat T-helper cells.
Preservative:
None present
This product
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.