Protein Name & Synonyms:
IgG concentration 1.0 mg/ml
This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.
This antibody may not be suitable for intracellular staining on some cell types.
This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.
This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.
Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Store at -20 °C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Quality Control (Contaminants):
Activated rat T-helper cells.
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.