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Antigen Information
Protein Name & Synonyms:
IgG2a
Target Species:
- Negative Control
Antibody Specifications
Applications:
Flow Cytometry
Concentration:
IgG concentration 0.1 mg/ml
Species Reactivity:
Negative Control
Specificity
This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.
This antibody may not be suitable for intracellular staining on some cell types.
This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.
This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.
Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
This antibody may not be suitable for intracellular staining on some cell types.
This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.
This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.
Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Formulation:
Phosphate buffered saline
Preparation
Immunogen:
Activated rat T-helper cells
Preservative:
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
This product
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
