Protein Name & Synonyms:
IgG2a
Applications:
Flow Cytometry
Reconstitution:
Reconstitute with 1.0 ml distilled water
Species Reactivity:
Negative Control
This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.
This antibody may not be suitable for intracellular staining on some cell types.
This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.
This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.
Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Prior to reconstitution store at +4 °C. Following reconstitution store at +4 °C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1.0 ml distilled water
Immunogen:
Activated rat T-helper cells.
Preservative:
0.09% | Sodium Azide |
1% | Bovine Serum Albumin |
5% | Sucrose |
This product
Spleen cells from BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.