Protein Name & Synonyms:
IgM concentration 0.1 mg/ml
This product can be used as a negative control for direct FITC immunofluorescence staining (e.g.. Flow Cytometry) when using mouse monoclonal antibodies to human cell surface antigens of isotype IgM. This enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections.
This antibody recognizes a rat cell surface marker and therefore cannot be used as a negative control in the species.
Store at +4 °C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Phosphate buffered saline
|1%||Bovine Serum Albumin|
Spleen cells from immunised BALB/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.