Protein Name & Synonyms:
Leishmania gp63
Applications:
ELISA (dilution: 1/500, 1/1000), Immunofluorescence
Reconstitution:
Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
Species Reactivity:
Protozoan
This product recognizes the Leishmania major surface protease (gp63), a 63kDa highly conserved protein which is found at high density on many Leishmania species. This product recognizes living and fixed parasites, but will not bind to denatured or improperly folded recombinant gp63.
Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Ascitic Fluid - lyophilized
Reconstitution:
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
Immunogen:
Recombinant baculovirus expressed L. major gp63
Preservative:
None present
This product
Spleen cells from immunised BALB/c mice were fused with cells of the X63Ag.653 myeloma cell line