Protein Name & Synonyms:
Flow Cytometry, Functional Assays
IgG concentration 1.0 mg/ml
This product recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. This product has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Quality Control (Contaminants):
Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.