Protein Name & Synonyms:
Immunoprecipitation (2-8 ug/ml), Flow cytometry (2-8 ug/ml), Immunohistochemistry (2-8 ug/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 ug/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.
PFP, P1 , FLH2 , HPLH2, PRF1, Cytolysin , MGC65093
Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
Purified granules from human YT lymphoma cells
100 µg (1 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol.
-20 °C. The shelf life is 12 months.