Mouse Anti-Pig Tubulin beta (N-term)

Mouse Anti-Pig Tubulin beta (N-term)

DS-MB-03517
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Antigen Information

Protein Name & Synonyms:
Tubulin beta
Target Species:
  • Porcine (pig)

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA (dilution: , 1.0ug/ml), Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Species Reactivity:
Pig, Human, Mouse, Plants, Rat

This product detects a band of approximately 50kDa in human RAJI and mouse EL4 cell lysates.

Specificity

This product recognizes an epitope within the N-terminal region (NT) of tubulin beta, a 50kDa globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape.

The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule.

This product is reported to partially inhibit microtubule assembly in vitro, see Draber, P. et al. for details. This product is reported as suitable for use in immunocytochemistry.

Storage/Stability

Store at +4 °C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgM - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Tubulin beta from porcine brain.
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.