Protein Name & Synonyms:
IgG concentration 0.1 mg/ml
This product recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).
This product is routinely tested in flow cytometry on rat splenocytes.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Phosphate buffered saline
PHA stimulated rat lymphocytes.
|1%||Bovine Serum Albumin|
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.