Protein Name & Synonyms:
Immunohistology - Frozen, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
This product does not require protein digestion pre-treatment of paraffin embedded sections.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
This product recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.
* This product been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde), see Whiteland, J.L. et al. for details.
This product is routinely tested in flow cytometry on rat splenocytes.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Rat thymocyte membrane glycoproteins.
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
12 months from the date of shipment when stored properly.