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Mouse Anti-Rat MHC Class I RT1A

Mouse Anti-Rat MHC Class I RT1A

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DS-MB-02244
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Antigen Information

Protein Name & Synonyms:
MHC Class I RT1A
Target Species:
  • Rat

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA (dilution: 1/50 , 1/100), Flow Cytometry, Immunoprecipitation
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Rat
Immunohistology:
Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.

Specificity

This product recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized.

This antibody has been used in immunoaffinity purification of rat MHC class I molecules.

This product is routinely tested in flow cytometry on rat splenocytes.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Rat spleen glycoproteins
Preservative:
0.09%Sodium Azide
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.