Protein Name & Synonyms:
MHC Class I RT1A
Immunohistology - Frozen, ELISA (dilution: 1/50 , 1/100), Flow Cytometry, Immunoprecipitation
IgG concentration 1.0 mg/ml
Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
This product recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized.
This antibody has been used in immunoaffinity purification of rat MHC class I molecules.
This product is routinely tested in flow cytometry on rat splenocytes.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Rat spleen glycoproteins
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.