Mouse Anti-Rat MHC Class II I-Ab,s [+RPE]

Mouse Anti-Rat MHC Class II I-Ab,s [+RPE]

$511.00
DS-MB-03446
+

Antigen Information

Protein Name & Synonyms:
MHC Class II I-Ab,s
Target Species:
  • Rat

Antibody Specifications

Applications:
Flow Cytometry
Reconstitution:
Reconstitute with 1 ml distilled water
Species Reactivity:
Rat, Mouse

Specificity

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region.

This product recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4).

This product also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.

This product is routinely tested in flow cytometry on Lewis rat splenocytes.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at +4 °C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 6 months after receipt.

Format

Supplied as:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1 ml distilled water

Preparation

Immunogen:
Rat thymocyte membrane glycoproteins.
Preservative:
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.