Protein Name & Synonyms:
MHC Class II I-Ak,s
Immunohistology - Frozen, Flow Cytometry, Immunohistology - Paraffin
IgG concentration 1.0 mg/ml
Rat, Mouse haplotypes k and s Does not recognize BDIX rat strain
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region.
This product recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.
The literature reports that this antibody reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region. for details.
This product is routinely tested in flow cytometry on rat splenocytes.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Rat thymocyte membrane glycoprotein
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.