Mouse Anti-Rat MHC Class II RT1B

Mouse Anti-Rat MHC Class II RT1B
CODE:  DS-MB-02257

Antigen Information

Protein Name & Synonyms:
MHC Class II RT1B
Target Species:
  • Rat

Antibody Specifications

Applications:
Immunohistology - Frozen, Flow Cytometry, Immunofluorescence, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Rat, Mouse haplotypes k and s Does not recognize BDIX rat strain

Specificity

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.

This product recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.

The literature reports that this antibody reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region.

This product is routinely tested in flow cytometry on rat splenocytes.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Rat thymocyte membrane glycoprotein
Preservative:
0.09%Sodium Azide
This product
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.