Mouse Anti-Rat MHC Class II RT1Bu

Mouse Anti-Rat MHC Class II RT1Bu


Antigen Information

Protein Name & Synonyms:
MHC Class II RT1Bu
Target Species:
  • Rat

Antibody Specifications

Immunohistology - Frozen, Flow Cytometry, Immunohistology - Paraffin
IgG concentration 1.0 mg/ml
Species Reactivity:
Rat, Mouse
This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.

This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.


The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.

This product recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4).

This product also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.

This product is routinely tested in flow cytometry on Lewis rat splenocytes.


Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.


Supplied as:
Purified IgG - liquid
Phosphate buffered saline
Format Type:


Rat thymocyte membrane glycoproteins
0.09% Sodium Azide (NaN3)
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.