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770-729-2992
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Product Specifications
Size:
0.1 mg
Protein Information
Protein Name & Synonyms:
MHC Class II RT1Bu
Target Species:
- Rat
Antibody Specifications
Applications:
Immunohistology - Frozen, Flow Cytometry, Immunohistology - Paraffin
Concentration (lot specific):
IgG concentration 1.0 mg/ml
Species Reactivity:
Rat, Mouse
Immunohistology:
This product does not require protein digestion pre-treatment of paraffin embedded sections.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
Specificity
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.
This product recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4).
This product also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.
This product is routinely tested in flow cytometry on Lewis rat splenocytes.
This product recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4).
This product also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.
This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Immunogen:
Rat thymocyte membrane glycoproteins
Preservative:
| 0.09% | Sodium Azide |
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
