Mouse Anti-Rat MHC Class II RT1Bu [+FITC]

Mouse Anti-Rat MHC Class II RT1Bu [+FITC]
CODE:  DS-MB-02264

Product Specifications

0.1 mg

Protein Information

Protein Name & Synonyms:
MHC Class II RT1Bu
Target Species:
  • Rat

Antibody Specifications

Flow Cytometry
Concentration (lot specific):
IgG concentration 0.1 mg/ml
Species Reactivity:
Rat, Mouse


The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.

This product recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4).

This product also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.

This product is routinely tested in flow cytometry on Lewis rat splenocytes.


Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.


Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Phosphate buffered saline


Rat thymocyte membrane glycoproteins
0.09%Sodium Azide
1%Bovine Serum Albumin
This product
Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
12 months from the date of shipment when stored properly.