Protein Name & Synonyms:
Immunohistology - Frozen, Immunofluorescence, Immunohistology - Paraffin, Western Blotting
IgG concentration 0.5mg/ml
This product reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts.For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
This product recognizes nestin, a large intermediate filament protein originally identified by Hockfield and McKay (1985) It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
TRIS buffered saline.
Nestin purified from embryonic rat spinal cord.
0.09% Sodium Azide (NaN3)
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line