Protein Name & Synonyms:
TCR alpha/beta
Applications:
Flow Cytometry
Concentration:
IgG concentration 0.1 mg/ml
Species Reactivity:
Rat, This antibody is reported to cross react with Cynomolgus monkey
This product recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Formulation:
Phosphate buffered saline
Immunogen:
Rat T Blasts and Erythrocytes
Preservative:
0.09% | Sodium Azide |
1% | Bovine Serum Albumin |
This product
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.